Will generate a trajectory using reCAT.

This method was wrapped inside a container. The original code of this method is available here.

ti_recat(TSPFold = 2, beginNum = 10, endNum = 15, step_size = 2,
  base_cycle_range_start = 6, base_cycle_range_end = 9,
  max_num = 300, clustMethod = "GMM", run_environment = NULL)

Arguments

TSPFold

integer; No documentation provided by authors (default: 2; range: from 2 to 10)

beginNum

integer; No documentation provided by authors (default: 10; range: from 2 to 20)

endNum

integer; No documentation provided by authors (default: 15; range: from 2 to 20)

step_size

integer; Determines the number of k to skip in your consensus path, ie ifstep_size = 2, then reCAT would only calculate and merge the paths fork = 12, 14, 16, 18, ..., n-2, n. We recommend step_size of up to a maximum of 5 while preserving the performance of reCAT. Usually a step_size of 2 (by default) would suffice and bigger steps are recommended for larger datasets (>1000 cells) in order to reduce computational time. (default: 2; range: from 2 to 20)

base_cycle_range_start

integer; The minimal number of four k's for computing the reference cycle mentioned in the manuscript. Can be set to 6 or 7 (default: 6; range: from 6 to 7)

base_cycle_range_end

integer; The maximal number of four k's for computing the reference cycle mentioned in the manuscript. Can be set to 6 or 7 (default: 9; range: from 9 to 10)

max_num

integer; No documentation provided by authors (default: 300; range: from 100 to 500)

clustMethod

discrete; No documentation provided by authors (default: "GMM"; values: "GMM", "Pam", "Kmeans")

run_environment

In which environment to run the method, can be "docker" or "singularity".

Value

A TI method wrapper to be used together with infer_trajectory

References

Liu, Z., Lou, H., Xie, K., Wang, H., Chen, N., Aparicio, O.M., Zhang, M.Q., Jiang, R., Chen, T., 2017. Reconstructing cell cycle pseudo time-series via single-cell transcriptome data. Nature Communications 8.